i-Taq™ PCR core Kit inhigh purity which is stable and effective for DNA amplification regardless the sort and reaction condition of template.
· Thermostable DNA polymerase in 94 Kda
· Taq DNA polymerase in high purity
Removal of protein originated from E.coli and DNA as PCR contaminant source.
· Optimized buffer to highly activate polymerase regardless the sort of template and reaction condition
· High reproducibility
· Various applications for cloned DNA and human genomic DNA, etc.
i-Taq™DNA Polymerase included in the product is the 94KDa thermostable DNA polymerase which is expressed in E.coli after cloning the polymerase gene of Thermusaquaticus(strain TY1). This removes protein originated from E.coli and DAN which can affect on PCR as contaminants to perform stable and effective DNA amplification and amplifies to maximum 5 Kb with genomic DNA or cDNA, etc.
i-Taq™DNA Polymerse is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. The enzyme provides a thermostability that meets the requirements of specialized PCR applications. A thermostable DNA polymerase exhibitsvery high activity in primer extension and other molecular biology application.
The product has the best polymerase activity and high reproducibility regardless of template or reaction condition by optimizing the composition of buffer.
The PCR products can be directly used for cloning of T-Vector and blunt-end vector after making blunt end.