Proof-reading removes mismatched nucleotide inserted under the polymerization of DNA polymerase and reform it to the matched nucleotide.
It helps to get more accurate PCR product and amplify long DNA template comparing to Taq DNA Polymerase.
Pfu DNA Polymerase provide the reliable test result so that it can be used for gene cloning and expression.
Maxime™ PCR PreMix Kit(i-pfu) has high fidelity and low error rate by such functions of i-pfu DNA polymerase.
It also has high stability and reproducibility and all components are premixed in one tube for the fast and simple test for various samples.
Dried & aliquoted PreMix Kit including high purity pfu DNA Polymerase which has proofreading function for gene cloning
- Dried & aliquoted PreMix, All-in-one product
All components for reaction in dried pellet in one tube
Direct use for PCR after adding template and primer
Gel loading dye included for Electrophoresis
- High stability and reproducibility
Dried & aliquoted preMix by ALHP(Automatic Liquid Handling Platform) system
Protection from oxidation and humidity by vacuum packaging
- High accuracy
Long DNA template amplification by proof-reading function
Application for gene cloning and expression
- Low error rate
3’→5’ exonuclease activity, 1×10-6 error rate
Blunt-end Cloning available.